| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huffer, Kelly S (15)
|
AZSAC |
1:03.66 |
1:02.99 |
-0.67 |
| 2 |
Taylor, Danielle M (17)
|
PCWCAB |
1:03.69 |
1:03.41 |
-0.28 |
| 3 |
Schultz, Caroline T (17)
|
INCSC |
1:03.17 |
1:03.78 |
0.61 |
| 4 |
Lowe, Carley A (15)
|
FLHIGH |
1:04.27 |
1:03.97 |
-0.30 |
| 5 |
Leach, Hannah M (18)
|
MTMAC |
1:06.70 |
1:04.05 |
-2.65 |
| 6 |
Deleske, Nora I (16)
|
CAGWSC |
1:02.99 |
1:04.18 |
1.19 |
| 7 |
Galyer, Alex J (17)
|
SCYSSC |
1:09.16 |
1:04.31 |
-4.85 |
| 8 |
Parker, Katherine E (17)
|
GASA |
1:05.12 |
1:04.35 |
-0.77 |
| 9 |
Gresser, Hanna M (16)
|
LEFAST |
1:04.21 |
1:04.48 |
0.27 |
| 10 |
Maguire, Marcella L (15)
|
CTRAC |
1:05.00 |
1:04.74 |
-0.26 |
| 11 |
Beil, Mallory J (15)
|
OHCLPR |
1:04.00 |
1:04.80 |
0.80 |
| 12 |
Berning, Margaret R (15)
|
OHDR |
1:03.39 |
1:04.95 |
1.56 |
| 13 |
Hayakawa, Lena C (18)
|
HIKSC |
1:05.01 |
1:05.01 |
0.00 |
| 14 |
Horomanski, Jessica L (17)
|
SCYSSC |
1:08.41 |
1:05.26 |
-3.15 |
| 15 |
Umbay, Deanne V (16)
|
CACSSC |
1:04.60 |
1:05.40 |
0.80 |
| 16 |
Kirkpatrick, Abigail D (16)
|
INSBSC |
1:05.29 |
1:05.62 |
0.33 |
| 17 |
Libang, Gabrielle A (18)
|
CACSSC |
1:04.49 |
1:05.63 |
1.14 |
| 18 |
Grout, Morgan O (16)
|
INCSC |
1:05.75 |
1:06.44 |
0.69 |
| 19 |
Aycock, Courtney T (18)
|
SETNAQ |
1:04.67 |
1:06.46 |
1.79 |
| 20 |
Ibrahim, Madison T (17)
|
STNTRO |
1:06.50 |
1:07.25 |
0.75 |
| 21 |
Strathman, Madison E (18)
|
MVTST |
1:08.90 |
1:07.57 |
-1.33 |
| 22 |
Tarazi, Valerie R (15)
|
ILBSC |
1:05.64 |
1:07.93 |
2.29 |
| 23 |
Blaauw, Hayley A (18)
|
CACSSC |
1:07.31 |
1:08.06 |
0.75 |
| 24 |
O'Leary, Bridget C (13)
|
ILBSC |
1:09.79 |
1:08.49 |
-1.30 |
|
Bonezzi, Jessica A (17)
|
LEFAST |
1:04.67 |
DQ |
- |